cd16 apc cy7 b73 1 Search Results


cd16 apc cy7 b73 1  (Revvity)
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Revvity cd16 apc cy7 b73 1
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cd3  (Miltenyi Biotec)
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Miltenyi Biotec cd3
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cd3 apc h7 sk 7 cd16 pe cy7 b73 1  (Miltenyi Biotec)
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mouse anti-cd16 apc cy7 b73.1  (Becton Dickinson)
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Mouse Anti Cd16 Apc Cy7 B73.1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multitesttm 6-color tbnk  (Becton Dickinson)
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Becton Dickinson multitesttm 6-color tbnk
<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
Multitesttm 6 Color Tbnk, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd45ro(uchl-1)  (Biotium)
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Biotium cd45ro(uchl-1)
<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
Cd45ro(Uchl 1), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd69-pe l78  (Becton Dickinson)
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<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
Cd69 Pe L78, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nkat2-pe dx27  (Becton Dickinson)
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<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
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cd19-allophycocyanin (apc) sj2sc1  (Becton Dickinson)
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<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
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cd45-percp 2d1  (Becton Dickinson)
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<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
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cd3-peridinin chlorophyll protein (percp) sk7  (Becton Dickinson)
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<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
Cd3 Peridinin Chlorophyll Protein (Percp) Sk7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd94-apc hp-3d9  (Becton Dickinson)
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<t> T-, B- and </t> NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.
Cd94 Apc Hp 3d9, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Immunity

Article Title: Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function

doi: 10.1016/j.immuni.2020.07.006

Figure Lengend Snippet:

Article Snippet: Mouse anti-CD16 APC Cy7 Clone B73.1 , BD Biosciences , Cat No. 561306; RRID: AB_10643005.

Techniques: Recombinant, Molecular Cloning, Blocking Assay, Conjugation Assay, Staining, Western Blot, Lysis, Mutagenesis, Isolation, Luminex, Transfection, Amplification, Software, Variant Assay

T-, B- and NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: T-, B- and NK-lymphocyte subpopulation relative and absolute counts at baseline in survivors and nonsurvivors.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques:

ROC analysis of T-lymphocyte subset absolute counts for in-hospital mortality and disease severity of COVID-19 patients. Receiver operating characteristic (ROC) curves are represented for total CD3+ and four subsets of T lymphocyte cells. Cutoff values were identified with the Youden’s index. ( A ) ROC analysis showing the performance of baseline absolute counts of total T lymphocyte and their subsets in distinguishing fatal cases from survivors. Cutoff values for: CD3+: 524 cells/µl, sensitivity 67,65%, specificity: 78.57%, AUC: 0.7850, p < 0.0001; CD3 + CD4+: < 369 cells/µl, sensitivity 76.47%, specificity: 75.40%, AUC: 0.7697, p < 0.0001; CD3 + CD8+: < 194 cells/µl, sensitivity 73.53%, specificity: 70.63%, AUC: 0.7800, p < 0.0001; CD3 + CD4 + CD8 + DP: < 6.5 cells/µl, sensitivity 73.53%, specificity: 73.02%, AUC: 0.7715, p < 0.0001; CD3 + CD4 − CD8 − DN: < 21.5 cells/µl, sensitivity 76.47%, specificity: 68.25%, AUC: 0.7674, p < 0.0001. ( B ) ROC analysis showing the performance of baseline absolute counts of total T lymphocytes and their subsets in distinguishing severe (NRM, NIV and OTI) from nonsevere (AA and VMK) cases. Cutoff value for: CD3+ : < 733 cells/µl, sensitivity 69.44%, specificity: 73.86%, AUC: 0.7295, p < 0.0001; CD3 + CD4+ : < 426 cells/µl, sensitivity 62.50%, specificity: 72.73%, AUC: 0.6862, p < 0.0001; CD3 + CD8+ : < 262 cells/µl, sensitivity 73.61%, specificity: 64.77%, AUC: 0.7483, p < 0.0001; CD3 + CD4 + CD8 + DP: < 4.5 cells/µl, sensitivity 41.67%, specificity: 90.91%, AUC: 0.7148, p < 0.0001; CD3 + CD4 − CD8 − DN: < 18.5 cells/µl, sensitivity 56.94%, specificity: 84.09%, AUC: 0.7319, p < 0.0001. AUC area under the curve, AA ambient air, VMK Venturi mask, NRM nonrebreather oxygen mask with concentrator, NIV noninvasive mechanical ventilation, OTI Orotracheal Intubation for mechanical ventilation.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: ROC analysis of T-lymphocyte subset absolute counts for in-hospital mortality and disease severity of COVID-19 patients. Receiver operating characteristic (ROC) curves are represented for total CD3+ and four subsets of T lymphocyte cells. Cutoff values were identified with the Youden’s index. ( A ) ROC analysis showing the performance of baseline absolute counts of total T lymphocyte and their subsets in distinguishing fatal cases from survivors. Cutoff values for: CD3+: 524 cells/µl, sensitivity 67,65%, specificity: 78.57%, AUC: 0.7850, p < 0.0001; CD3 + CD4+: < 369 cells/µl, sensitivity 76.47%, specificity: 75.40%, AUC: 0.7697, p < 0.0001; CD3 + CD8+: < 194 cells/µl, sensitivity 73.53%, specificity: 70.63%, AUC: 0.7800, p < 0.0001; CD3 + CD4 + CD8 + DP: < 6.5 cells/µl, sensitivity 73.53%, specificity: 73.02%, AUC: 0.7715, p < 0.0001; CD3 + CD4 − CD8 − DN: < 21.5 cells/µl, sensitivity 76.47%, specificity: 68.25%, AUC: 0.7674, p < 0.0001. ( B ) ROC analysis showing the performance of baseline absolute counts of total T lymphocytes and their subsets in distinguishing severe (NRM, NIV and OTI) from nonsevere (AA and VMK) cases. Cutoff value for: CD3+ : < 733 cells/µl, sensitivity 69.44%, specificity: 73.86%, AUC: 0.7295, p < 0.0001; CD3 + CD4+ : < 426 cells/µl, sensitivity 62.50%, specificity: 72.73%, AUC: 0.6862, p < 0.0001; CD3 + CD8+ : < 262 cells/µl, sensitivity 73.61%, specificity: 64.77%, AUC: 0.7483, p < 0.0001; CD3 + CD4 + CD8 + DP: < 4.5 cells/µl, sensitivity 41.67%, specificity: 90.91%, AUC: 0.7148, p < 0.0001; CD3 + CD4 − CD8 − DN: < 18.5 cells/µl, sensitivity 56.94%, specificity: 84.09%, AUC: 0.7319, p < 0.0001. AUC area under the curve, AA ambient air, VMK Venturi mask, NRM nonrebreather oxygen mask with concentrator, NIV noninvasive mechanical ventilation, OTI Orotracheal Intubation for mechanical ventilation.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques:

Lymphocyte subset absolute counts in the five groups of patients stratified according to maximal oxygen supply/ventilation support. Absolute counts of total T-lymphocytes (CD3+, ( A ) and related subsets (CD3 + CD4+, ( B ) CD3 + CD8+, ( C ) CD3 + CD4 + CD8 + DP, ( D ) CD3 + CD4 − CD8 − DN, ( E ) CD4/CD8 ratio ( F ) B-lymphocytes (CD19+, ( G ) and NK-cells (CD3 neg CD16 + CD56+, ( H ) in the study population after stratification according to maximal oxygen supply/ventilation support required during hospitalization. Differences were analyzed using the Kruskal–Wallis and Dunn’s multiple comparison tests; the AA group was used as a reference group for multiple comparisons. Patients were further grouped into nonsevere (AA and VMK, blue dashed line box) and severe (NRM, NIV and OTI, red dashed line box); differences were analyzed using the Mann–Whitney test. A two-sided p value of < 0.05 was considered statistically significant. AA ambient air, VMK Venturi mask, NRM nonrebreather oxygen mask with concentrator, NIV noninvasive mechanical ventilation, OTI Orotracheal Intubation for mechanical ventilation. *0.01 < p < 0.5; **0.001 < p < 0.01; ***0.0001 < p < 0.00001; ****p < 0.0001.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: Lymphocyte subset absolute counts in the five groups of patients stratified according to maximal oxygen supply/ventilation support. Absolute counts of total T-lymphocytes (CD3+, ( A ) and related subsets (CD3 + CD4+, ( B ) CD3 + CD8+, ( C ) CD3 + CD4 + CD8 + DP, ( D ) CD3 + CD4 − CD8 − DN, ( E ) CD4/CD8 ratio ( F ) B-lymphocytes (CD19+, ( G ) and NK-cells (CD3 neg CD16 + CD56+, ( H ) in the study population after stratification according to maximal oxygen supply/ventilation support required during hospitalization. Differences were analyzed using the Kruskal–Wallis and Dunn’s multiple comparison tests; the AA group was used as a reference group for multiple comparisons. Patients were further grouped into nonsevere (AA and VMK, blue dashed line box) and severe (NRM, NIV and OTI, red dashed line box); differences were analyzed using the Mann–Whitney test. A two-sided p value of < 0.05 was considered statistically significant. AA ambient air, VMK Venturi mask, NRM nonrebreather oxygen mask with concentrator, NIV noninvasive mechanical ventilation, OTI Orotracheal Intubation for mechanical ventilation. *0.01 < p < 0.5; **0.001 < p < 0.01; ***0.0001 < p < 0.00001; ****p < 0.0001.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques: MANN-WHITNEY

Univariable and multivariable logistic regression analysis for in-hospital mortality-related risks in patients with SARS-CoV-2 infection.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: Univariable and multivariable logistic regression analysis for in-hospital mortality-related risks in patients with SARS-CoV-2 infection.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques: Infection

Univariable and multivariable logistic regression analysis for severity-related risks in patients with SARS-CoV-2 infection.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: Univariable and multivariable logistic regression analysis for severity-related risks in patients with SARS-CoV-2 infection.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques: Infection

Correlation between lymphocyte subpopulation absolute counts and inflammation markers. Absolute counts of total T-lymphocytes and related subsets (CD3 + CD4+, CD3 + CD8+, CD3 + CD4 + CD8 + DP, CD3 + CD4 − CD8 − DN), B-lymphocytes and NK-cells (CD16 + CD56+) were correlated with IL-6 (column A), CRP serum concentrations (column B) and d -dimer plasma concentration (column C). Correlation was assessed with the Spearman’s test; Spearman r (only if statistically significant) and p are reported in the boxes; a two-sided p value of < 0.05 was considered statistically significant. ns not statistically significant, IL-6 interleukin-6, CRP C-reactive protein.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: Correlation between lymphocyte subpopulation absolute counts and inflammation markers. Absolute counts of total T-lymphocytes and related subsets (CD3 + CD4+, CD3 + CD8+, CD3 + CD4 + CD8 + DP, CD3 + CD4 − CD8 − DN), B-lymphocytes and NK-cells (CD16 + CD56+) were correlated with IL-6 (column A), CRP serum concentrations (column B) and d -dimer plasma concentration (column C). Correlation was assessed with the Spearman’s test; Spearman r (only if statistically significant) and p are reported in the boxes; a two-sided p value of < 0.05 was considered statistically significant. ns not statistically significant, IL-6 interleukin-6, CRP C-reactive protein.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques: Concentration Assay

T-, B- and NK-lymphocyte subpopulation absolute counts at baseline after patient stratification according to maximal oxygen supply/ventilation support.

Journal: Scientific Reports

Article Title: Baseline T-lymphocyte subset absolute counts can predict both outcome and severity in SARS-CoV-2 infected patients: a single center study

doi: 10.1038/s41598-021-90983-0

Figure Lengend Snippet: T-, B- and NK-lymphocyte subpopulation absolute counts at baseline after patient stratification according to maximal oxygen supply/ventilation support.

Article Snippet: Lymphocyte subpopulations were assessed using BD Multitest™ 6-color TBNK (FITC-labeled CD3 clone SK7; PE-labeled CD16, clone B73.1, and CD56, clone NCAM 16.2; PerCP-Cy™5.5-labeled CD45, clone 2D1; PE-Cy™7-labeled CD4, clone SK3; APC-labeled CD19, clone SJ25C1; APC-Cy7-labeled CD8, clone SK1) and BD Trucount™ tubes for absolute count with a lyse-no-wash procedure (BD FACS™Lysing Solution).

Techniques: